If you have a basket of fruit filled with oranges and plums, and plums weigh about half what the oranges weigh. But if you use the plums for standardization then you will count the number of fruit in the basket and weigh only one plum and multiply it by the number of fruit in the basket. Not taking in consideration the difference in weight between the oranges and plums.
These scientists who support HPLC count all the fruit as if they are plums. Since plums are on average about half the weight of oranges the measurement ends up being less than half of what it actually should be. This is because in olive oil there is no significant amount of hydroxytyrosol and tyrosol. If you detect any significant amount of hydroxytyrosol and tyrosol in olive oil it means the olive oil is rancid. This is why hydroxytyrosol and tyrosol used for supplements are not extracted from olive oil they are extracted from the stinking rotten waste waters of olive mills.
These phenolic compounds extracted from olive mill waste waters (hydroxytyrosol, tyrosol) do not have the same health benefit as phenolic compounds (oleocanthal, oleacein) that are found in significant amounts in early harvest olive oil made form specific olive tree varieties.
These scientists refuse to acknowledge this basic knowledge and facts of chemistry and yet they have the responsibility to clarify how to measure polyphenols in olive oil for implementing the health claim.
In recent years a new wave of young Greek and Cypriot olive growers have revived this ancient Hellenic quality early harvest EVOO and are making it available to the world.
I think that this issue was commented, at my request, in the Baena Congress we met. I sent a sample to the EUROLIVE oils to Prof. Prokopios. He compared the values and wrote me that the values were comparable.
Dr. M.I. Covas
The only official method for measuring phenolic compounds of EVOO to substantiate the EU 432/2012 health claim and place it on the label is HPLC-MS/MS or any other method whose measurement matches it namely the NMR method. The NMR has the added advantage of measuring each phenolic compound in seconds without the use of any chemical reagents.Dr. Prokopios Magiatis and Dr. Eleni Melliou along with a team of researchers developed the NMR method at the Univ of Athens in 2012. They also invented the Aristoleo Test Kit, “lab in a vial” and the “Aristometro” portable spectrometer, and the “Predictor” a handheld portable olive mill.
The Aristometro, and the Predictor were created under the Interreg MED programme
ARISTOIL: “Reinforcement of Mediterranean olive oil sector competitiveness through development and application of innovative production and quality control methodologies related to olive oil health protecting properties”
INTERREG MED PROGRAMME (2014 – 2020)